Composite
Part:BBa_K5103001:Experience
Designed by: Nikoo Mansourian Group: iGEM24_Guelph (2024-09-17)
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Applications of BBa_K5103001
We did not have much trouble creating this part and it was successfully transformed into E. coli DH5a, but we failed in transforming it into Bacillus subtilis.
Figure 1. 0.8% Agarose gel electrophoresis of PCG-CRY plasmid digested with BsaI compared to the undigested PCG-CRY plasmid. Digestion shows successful ligation of the Cry8Da gene in pCG004 plasmid backbone. Results showed a band at ~8000bp corresponding with PCG004 and ~3400 bp corresponding with Cry8Da.
User Reviews
UNIQ3ae9db8a600c5bc1-partinfo-00000000-QINU UNIQ3ae9db8a600c5bc1-partinfo-00000001-QINU